Logo Logo
Switch Language to German
Beller, Ebba; Reuter, Laura; Kluge, Anne; Preibisch, Christine; Lindauer, Ute; Bogdanov, Alexei; Laemmer, Friederike; Delbridge, Claire; Matiasek, Kaspar; Schwaiger, Benedikt J.; Böckh-Behrens, Tobias; Zimmer, Claus; Gersing, Alexandra S. (2018): Pilot study to assess visualization and therapy of inflammatory mechanisms after vessel reopening in a mouse stroke model. In: Scientific Reports, Vol. 8, 745
Full text not available from 'Open Access LMU'.


After reperfusion therapy in stroke patients secondary inflammatory processes may increase cerebral damage. In this pilot study, effects of anti-inflammatory therapy were assessed in a middle cerebral artery occlusion (MCAO) mouse model after reperfusion. 1 hour after MCAO, the artery was reopened and tacrolimus or NaCl were administered intra-arterially. Perfusion-weighted (PWI) and diffusion-weighted images (DWI) were obtained by MRI during MCAO. DWI, T2- and T1-weighted images with and without Bis-5HT-DTPA administration were obtained 24 hours after MCAO. Neutrophils, Myeloperoxidase-positive-(MPO+)-cells and microglia, including M1 and M2 phenotypes, were assessed immunohistochemically. Treatment with tacrolimus led to significantly smaller apparent diffusion coefficient (ADC) lesion volume within 24 hours (median -55.6mm(3), range -81.3 to -3.6, vs. median 8.0 mm(3), range 1.2 to 41.0;P = 0.008) and significantly lower enhancement of Bis-5-HT-DTPA (median signal intensity (SI) ratio(cortex), median 92.0%, range 82.8% to 97.1%, vs. median 103.1%, range 98.7% to 104.6%;P = 0.008) compared to the NaCl group. Immunohistochemical analysis showed no significant differences between both groups. Intra-arterially administered anti-inflammatory agents after mechanical thrombectomy may improve treatment efficiency in stroke by reducing infarct volume size and MPO activity.