Abstract
After reperfusion therapy in stroke patients secondary inflammatory processes may increase cerebral damage. In this pilot study, effects of anti-inflammatory therapy were assessed in a middle cerebral artery occlusion (MCAO) mouse model after reperfusion. 1 hour after MCAO, the artery was reopened and tacrolimus or NaCl were administered intra-arterially. Perfusion-weighted (PWI) and diffusion-weighted images (DWI) were obtained by MRI during MCAO. DWI, T2- and T1-weighted images with and without Bis-5HT-DTPA administration were obtained 24 hours after MCAO. Neutrophils, Myeloperoxidase-positive-(MPO+)-cells and microglia, including M1 and M2 phenotypes, were assessed immunohistochemically. Treatment with tacrolimus led to significantly smaller apparent diffusion coefficient (ADC) lesion volume within 24 hours (median -55.6mm(3), range -81.3 to -3.6, vs. median 8.0 mm(3), range 1.2 to 41.0;P = 0.008) and significantly lower enhancement of Bis-5-HT-DTPA (median signal intensity (SI) ratio(cortex), median 92.0%, range 82.8% to 97.1%, vs. median 103.1%, range 98.7% to 104.6%;P = 0.008) compared to the NaCl group. Immunohistochemical analysis showed no significant differences between both groups. Intra-arterially administered anti-inflammatory agents after mechanical thrombectomy may improve treatment efficiency in stroke by reducing infarct volume size and MPO activity.
Item Type: | Journal article |
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Faculties: | Veterinary Medicine |
Subjects: | 600 Technology > 610 Medicine and health |
URN: | urn:nbn:de:bvb:19-epub-65863-8 |
ISSN: | 2045-2322 |
Language: | English |
Item ID: | 65863 |
Date Deposited: | 19. Jul 2019, 12:18 |
Last Modified: | 04. Nov 2020, 13:46 |