Improved Ribo-seq enables identification of cryptic translation events

Erhard, Florian; Halenius, Anne; Zimmermann, Cosima; L'Hernault, Anne; Kowalewski, Daniel J.; Weekes, Michael P.; Stevanovic, Stefan; Zimmer, Ralf und Doelken, Lars (2018): Improved Ribo-seq enables identification of cryptic translation events. In: Nature Methods, Bd. 15, Nr. 5: S. 363-366

Abstract

Ribosome profiling has been used to predict thousands of short open reading frames (sORFs) in eukaryotic cells, but it suffers from substantial levels of noise. PRICE (https://github.com/erhard-lab/price) is a computational method that models experimental noise to enable researchers to accurately resolve overlapping sORF s and noncanonical translation initiation. We experimentally validated translation using major histocompatibility complex class I (MHC I) peptidomics and observed that sORF-derived peptides efficiently enter the MHC I presentation pathway and thus constitute a substantial fraction of the antigen repertoire.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Mathematik, Informatik und Statistik > Informatik
• Themengebiete: 000 Informatik, Informationswissenschaft, allgemeine Werke > 004 Informatik
• ISSN: 1548-7091
• Sprache: Englisch