Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging

Strauss, Sebastian; Nickels, Philipp C.; Strauss, Maximilian T.; Sabinina, Vilma Jimenez; Ellenberg, Jan; Carter, Jeffrey D.; Gupta, Shashi; Janjic, Nebojsa und Jungmann, Ralf (2018): Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging. In: Nature Methods, Bd. 15, Nr. 9: S. 685-688

Abstract

Although current implementations of super-resolution microscopy are technically approaching true molecular-scale resolution, this has not translated to imaging of biological specimens, because of the large size of conventional affinity reagents. Here we introduce slow off-rate modified aptamers (SOMAmers) as small and specific labeling reagents for use with DNA points accumulation in nanoscale topography (DNA-PAINT). To demonstrate the achievable resolution, specificity, and multiplexing capability of SOMAmers, we labeled and imaged both transmembrane and intracellular targets in fixed and live cells.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Physik
• Themengebiete: 500 Naturwissenschaften und Mathematik > 530 Physik
• ISSN: 1548-7091
• Sprache: Englisch