Template-free 2D particle fusion in localization microscopy

Heydarian, Hamidreza; Schüder, Florian; Strauss, Maximilian T.; Werkhoven, Ben van; Fazel, Mohamadreza; Lidke, Keith A.; Jungmann, Ralf; Stallinga, Sjoerd und Rieger, Bernd (2018): Template-free 2D particle fusion in localization microscopy. In: Nature Methods, Bd. 15, Nr. 10: S. 781-784

Abstract

Methods that fuse multiple localization microscopy images of a single structure can improve signal-to-noise ratio and resolution, but they generally suffer from template bias or sensitivity to registration errors. We present a template-free particle-fusion approach based on an all-to-all registration that provides robustness against individual misregistrations and underlabeling. We achieved 3.3-nm Fourier ring correlation (FRC) image resolution by fusing 383 DNA origami nanostructures with 80% labeling density, and 5.0-nm resolution for structures with 30% labeling density.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Physik
• Themengebiete: 500 Naturwissenschaften und Mathematik > 530 Physik
• ISSN: 1548-7091
• Sprache: Englisch