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Heydarian, Hamidreza; Schüder, Florian; Strauss, Maximilian T.; Werkhoven, Ben van; Fazel, Mohamadreza; Lidke, Keith A.; Jungmann, Ralf; Stallinga, Sjoerd and Rieger, Bernd (2018): Template-free 2D particle fusion in localization microscopy. In: Nature Methods, Vol. 15, No. 10: pp. 781-784

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Methods that fuse multiple localization microscopy images of a single structure can improve signal-to-noise ratio and resolution, but they generally suffer from template bias or sensitivity to registration errors. We present a template-free particle-fusion approach based on an all-to-all registration that provides robustness against individual misregistrations and underlabeling. We achieved 3.3-nm Fourier ring correlation (FRC) image resolution by fusing 383 DNA origami nanostructures with 80% labeling density, and 5.0-nm resolution for structures with 30% labeling density.

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