Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures

Manuguerra, Ilenia; Croce, Stefano; El-Sagheer, Afaf H.; Krissanaprasit, Abhichart; Brown, Tom; Gothelf, Kurt V. und Manetto, Antonio (2018): Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures. In: Chemical Communications, Bd. 54, Nr. 36: S. 4529-4532

Abstract

Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Chemie und Pharmazie > Department Chemie
• Themengebiete: 500 Naturwissenschaften und Mathematik > 540 Chemie
• ISSN: 1359-7345
• Sprache: Englisch