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Manuguerra, Ilenia; Croce, Stefano; El-Sagheer, Afaf H.; Krissanaprasit, Abhichart; Brown, Tom; Gothelf, Kurt V.; Manetto, Antonio (2018): Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures. In: Chemical Communications, Vol. 54, No. 36: pp. 4529-4532
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Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.