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Robert, Hadrien M. L.; Savatier, Julien; Vial, Stephanie; Verghese, Jacob; Wattellier, Benoit; Rigneault, Herve; Monneret, Serge; Polleux, Julien; Baffou, Guillaume (2018): Photothermal Control of Heat-Shock Protein Expression at the Single Cell Level. In: Small, Vol. 14, No. 32, 1801910
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Abstract

Laser heating of individual cells in culture recently led to seminal studies in cell poration, fusion, migration, or nanosurgery, although measuring the local temperature increase in such experiments remains a challenge. Here, the laser-induced dynamical control of the heat-shock response is demonstrated at the single cell level, enabled by the use of light-absorbing gold nanoparticles as nanosources of heat and a temperature mapping technique based on quadriwave lateral shearing interferometry (QLSI) measurements. As it is label-free, this approach does not suffer from artifacts inherent to previously reported fluorescence-based temperature-mapping techniques and enables the use of any standard fluorescent labels to monitor in parallel the cell's response.