Abstract
Laser heating of individual cells in culture recently led to seminal studies in cell poration, fusion, migration, or nanosurgery, although measuring the local temperature increase in such experiments remains a challenge. Here, the laser-induced dynamical control of the heat-shock response is demonstrated at the single cell level, enabled by the use of light-absorbing gold nanoparticles as nanosources of heat and a temperature mapping technique based on quadriwave lateral shearing interferometry (QLSI) measurements. As it is label-free, this approach does not suffer from artifacts inherent to previously reported fluorescence-based temperature-mapping techniques and enables the use of any standard fluorescent labels to monitor in parallel the cell's response.
Item Type: | Journal article |
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Research Centers: | Center for NanoScience (CENS) |
Subjects: | 500 Science > 500 Science |
ISSN: | 1613-6810 |
Language: | English |
Item ID: | 68032 |
Date Deposited: | 19. Jul 2019, 12:23 |
Last Modified: | 14. Dec 2021, 08:55 |