Tyrosine-1 of RNA Polymerase II CTD Controls Global Termination of Gene Transcription in Mammals

Shah, Nilay; Maqbool, Muhammad Ahmad; Yahia, Yousra; El Aabidine, Amal Zine; Esnault, Cyril; Forne, Ignasi; Decker, Tim-Michael; Martin, David; Schüller, Roland; Krebs, Stefan; Blum, Helmut; Imhof, Axel; Eick, Dirk und Andrau, Jean-Christophe (2018): Tyrosine-1 of RNA Polymerase II CTD Controls Global Termination of Gene Transcription in Mammals. In: Molecular Cell, Bd. 69, Nr. 1: S. 48-61

Abstract

The carboxy-terminal domain (CTD) of RNA polymerase (Pol) II is composed of a repetition of YSPTSPS heptads and functions as a loading platform for protein complexes that regulate transcription, splicing, and maturation of RNAs. Here, we studied mammalian CTD mutants to analyze the function of tyrosine1 residues in the transcription cycle. Mutation of 3/4 of the tyrosine residues (YFFF mutant) resulted in a massive read-through transcription phenotype in the antisense direction of promoters as well as in the 30 direction several hundred kilobases downstream of genes. The YFFF mutant shows reduced Pol II at promoterproximal pause sites, a loss of interaction with the Mediator and Integrator complexes, and impaired recruitment of these complexes to chromatin. Consistent with these observations, Pol II loading at enhancers and maturation of snRNAs are altered in the YFFF context genome-wide. We conclude that tyrosine1 residues of the CTD control termination of transcription by Pol II.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Fakultätsübergreifende Einrichtungen: Center for Integrated Protein Science Munich (CIPSM)
• Themengebiete: 500 Naturwissenschaften und Mathematik > 540 Chemie ; 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 1097-2765
• Sprache: Englisch