Ertl, H.; Gerlich, W.; Koszinowski, Ulrich H.
Detection of antibodies to Sendai virus by enzyme-linked immunosorbent assay (ELISA).
In: Journal of Immunological Methods, Vol. 28: pp. 163-176
An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Sendai virus, a paramyxovirus, is described. The assay was found to be about 20-fold more sensitive than the hemagglutination inhibition assay. Differentiation between virus specific IgG and IgM is possible. The test appears to be especially useful in the study of early events in antibody formation in vivo as well as in vitro.
Abbreviations: BSA, bovine serum albumin; CF, chorioallantoic fluid; ChHC, chicken host components; ELISA, enzyme-linked immunosorbent assay; FCS, fetal calf serum; HAU, hemagglutinating units; HAIU, hemagglutination inhibition units; HIA, hemagglutination inhibition assay; HRPO, horse radish peroxidase; i.n., intranasal; i.p., intraperitoneal; i.v., intravenous; M, molar; A, absorbance; PBS, phosphate buffered saline; RAM/Ig, rabbit anti-mouse-Ig; RAM/IgG, rabbit anti-mouse-IgG; RAM/IgM, rabbit anti-mouse-IgM (Fc); RIA, radioimmunoassay; T-cells, thymus derived cells; TCID50, 50% tissue culture infective doses; s.c., subcutaneous; SV, Sendai virus; w/v, weight per volume