Tynan, Katherine; Olsen, Anne; Trask, Barbara; Jong, Pieter de; Thompson, John A.; Zimmermann, Wolfgang; Carrano, Anthony; Mohrenweiser, Harvey
Assembly and Analysis of Cosmid Contigs in the CEA-gene Family Region of Human Chromosome 19.
In: Nucleic Acids Research, Vol. 20, No. 7: pp. 1629-1636
The carcinoembryonic antigen (CEA)-like genes are members of a large gene family which Is part of the immunoglobulln superfamily. The CEA family is divided into two major subgroups, the CEA-subgroup and the pregnancy-specific glycoprotein (PSG)-subgroup. In the course of an effort to develop a set of overlapping cosmlds spanning human chromosome 19, we identified 245 cosmids In a human chromosome 19 cosmid library (6 – 7X redundant) by hybridization with an IgC-like domain fragment of the CEA gene. A fluorescence-based restriction enzyme digest fingerprinting strategy was used to assemble 212 probe-positive cosmids, along with 115 additional cosmids from a collection of ~ 8, 000 randomly selected cosmids, into five contigs. Two of the contigs contain CEA-subgroup genes while the remaining three contigs contain PSG-subgroup genes. These five contigs range in size from 100 kb to over 300 kb and span an estimated 1 Mb. The CEA-like gene family was determined by fluorescence in situ hybridization to map in the q13.1-q13.2 region of human chromosome 19. Analysis of the two CEA-subgroup contigs provided verification of the contig assembly strategy and insight into the organization of 9 CEA-subgroup genes.