Synthesis of an acp3U phosphoramidite and incorporation of the hypermodified base into RNA

Nainyte, Milda ; Amatov, Tynchtyk ; Carell, Thomas (17. September 2019): Synthesis of an acp3U phosphoramidite and incorporation of the hypermodified base into RNA. In: Chemical Communications, Vol. 55: pp. 12216-12218

Abstract

acp3U is a hypermodified base that is found in the tRNAs of prokaryotes and eukaryotes and also in the ribosomal RNA of mammals. Its function has so far been unknown but it is speculated that acp3U complexes Mg ions, which may contribute to the stabilization of the RNA structure. As a hypermodified base in which a nucleoside is covalently connected to an amino acid, acp3U is a natural nucleoside between genotype and phenotype and hence is also of particular importance for theories about the origin of life. Herein, we report the development of a phosphoramidite building block and of a solid phase protocol that allows synthesis of RNA containing acp3U.

• Item Type: Journal article
• EU Funded Grant Agreement Number: 741912
• EU Projects: Horizon 2020 > ERC Grants > ERC Advanced Grant > ERC Grant 741912: EPiR - The Chemical Basis of RNA Epigenetics
• Form of publication: Postprint
• Faculties: Chemistry and Pharmacy > Department of Chemistry
• Research Centers: Center for Integrated Protein Science Munich (CIPSM)
• Subjects: 500 Science > 540 Chemistry
• ISSN: 0009-241X; 0022-4936; 1359-7345; 1364-548X
• Language: English

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