Puromycin Sensitivity of Ribosomal Label after Incorporation of 14C-Labelled Amino Acids into Isolated Mitochondria from Neurospora crassa

Neupert, Walter; Sebald, Walter; Schwab, Andreas J.; Pfaller, A. and Bücher, Theodor (October 1969): Puromycin Sensitivity of Ribosomal Label after Incorporation of 14C-Labelled Amino Acids into Isolated Mitochondria from Neurospora crassa. In: European Journal of Biochemistry, Vol. 10, No. 3: pp. 585-588

Abstract

Radioactive amino acids were incorporated into isolated mitochondria from Neurospora crassa. Then the mitochondrial ribosomes were isolated and submitted to density gradient centrifugation. A preferential labelling of polysomes was observed. However, when the mitochondrial suspension was treated with puromycin after amino acid incorporation, no radioactivity could be detected in either the monosomes or the polysomes. The conclusion is drawn that isolated mitochondria under these conditions do not incorporate significant amounts of amino acids into proteins of their ribosomes.

• Item Type: Journal article
• Faculties: Medicine
• Subjects: 600 Technology > 610 Medicine and health
• ISSN: 1432-1033