Protein-coding variants contribute to the risk of atopic dermatitis and skin-specific gene expression

Mucha, Sören; Ahluwalia, Tarunveer S.; Bisgaard, Hans; Peters, Annette; Gieger, Christian; Waldenberger, Melanie; Schulz, Holger; Strauch, Konstantin; Werfel, Thomas; Lee, Young-Ae; Wolfien, Markus; Rosenstiel, Philip; Wolkenhauer, Olaf; Schreiber, Stefan; Franke, Andre; Weidinger, Stephan und Ellinghaus, David (2020): Protein-coding variants contribute to the risk of atopic dermatitis and skin-specific gene expression. In: The Journal of Allergy and Clinical Immunology, Bd. 145, Nr. 4: S. 1208-1218

Abstract

BACKGROUND Fifteen percent of atopic dermatitis (AD) liability-scale heritability could be attributed to 31 susceptibility loci identified by using genome-wide association studies, with only 3 of them (IL13, IL-6 receptor IL6R, and filaggrin FLG) resolved to protein-coding variants. OBJECTIVE We examined whether a significant portion of unexplained AD heritability is further explained by low-frequency and rare variants in the gene-coding sequence. METHODS We evaluated common, low-frequency, and rare protein-coding variants using exome chip and replication genotype data of 15,574 patients and 377,839 control subjects combined with whole-transcriptome data on lesional, nonlesional, and healthy skin samples of 27 patients and 38 control subjects. RESULTS An additional 12.56{\%} (SE, 0.74{\%}) of AD heritability is explained by rare protein-coding variation. We identified docking protein 2 (DOK2) and CD200 receptor 1 (CD200R1) as novel genome-wide significant susceptibility genes. Rare coding variants associated with AD are further enriched in 5 genes (IL-4 receptor IL4R, IL13, Janus kinase 1 JAK1, JAK2, and tyrosine kinase 2 TYK2) of the IL13 pathway, all of which are targets for novel systemic AD therapeutics. Multiomics-based network and RNA sequencing analysis revealed DOK2 as a central hub interacting with, among others, CD200R1, IL6R, and signal transducer and activator of transcription 3 (STAT3). Multitissue gene expression profile analysis for 53 tissue types from the Genotype-Tissue Expression project showed that disease-associated protein-coding variants exert their greatest effect in skin tissues. CONCLUSION Our discoveries highlight a major role of rare coding variants in AD acting independently of common variants. Further extensive functional studies are required to detect all potential causal variants and to specify the contribution of the novel susceptibility genes DOK2 and CD200R1 to overall disease susceptibility.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin > Institut für Medizinische Informationsverarbeitung, Biometrie und Epidemiologie
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• Bemerkung: Vollständige Autorenliste s. Volltext
• Sprache: Englisch