Intra- and interspecific polymorphisms ofLeishmania donovani andL. tropica minicircle DNA

Dobner, P.; Löscher, Thomas and Rinder, H. (1994): Intra- and interspecific polymorphisms ofLeishmania donovani andL. tropica minicircle DNA. In: Parasitology Research, Vol. 80, No. 6: pp. 474-477

Abstract

A pair of degenerate polymerase chain reaction (PCR) primers (LEI-1, TCG GAT CC[C,T] [G,C]TG GGT AGG GGC GT; LEI-2, ACG GAT CC[G,C] [G,C][A,C]C TAT [A,T]TT ACA CC) defining a 0.15-kb segment ofLeishmania minicircle DNA was constructed. These primers amplified not only inter- but also intraspecifically polymorphic sequences. Individual sequences revealed a higher intraspecific than interspecific divergence. It is concluded that individual sequences are of limited relevance for species determination. In contrast, when a data base of 19 different sequences was analyzed in a dendrographic plot, an accurate species differentiation was feasible.

• Item Type: Journal article
• Faculties: Medicine
• Subjects: 600 Technology > 610 Medicine and health
• ISSN: 1432-1955