New simplified molecular design for functional T cell receptor

Brocker, Thomas; Peter, Annick; Traunecker, Andre and Karjalainen, Klaus (1993): New simplified molecular design for functional T cell receptor. In: European Journal of Immunology, Vol. 23, No. 7: pp. 1435-1439

Abstract

We have produced a chimeric single-chain T cell receptor (TcR) that combines the specific antibody recognition function and TcR/CD3 signaling properties within the same polypeptide chain. This hybrid molecule consisted of a single-chain antibody combining site that was connected over a short spacer to the transmembrane and cytoplasmic region of CD3. When expressed on TcR- or TcR+ T cell hybridomas it could mediate recognition of relevent target cells and subsequent production of lymphokines; i.e. it could functionally replace the TcR/CD3 complex. Therefore, the single-chain TcR model presented here represents an interesting and useful means for the creation of T cells with new specificities.

• Item Type: Journal article
• Keywords: T cell receptor, Single-chain variable regions
• Faculties: Medicine
• Subjects: 600 Technology > 610 Medicine and health
• ISSN: 1521-4141