
Abstract
We have produced a chimeric single-chain T cell receptor (TcR) that combines the specific antibody recognition function and TcR/CD3 signaling properties within the same polypeptide chain. This hybrid molecule consisted of a single-chain antibody combining site that was connected over a short spacer to the transmembrane and cytoplasmic region of CD3. When expressed on TcR- or TcR+ T cell hybridomas it could mediate recognition of relevent target cells and subsequent production of lymphokines; i.e. it could functionally replace the TcR/CD3 complex. Therefore, the single-chain TcR model presented here represents an interesting and useful means for the creation of T cells with new specificities.
Item Type: | Journal article |
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Keywords: | T cell receptor, Single-chain variable regions |
Faculties: | Medicine |
Subjects: | 600 Technology > 610 Medicine and health |
URN: | urn:nbn:de:bvb:19-epub-7669-4 |
ISSN: | 1521-4141 |
Item ID: | 7669 |
Date Deposited: | 20. Nov 2008, 12:10 |
Last Modified: | 04. Nov 2020, 12:50 |