The immunohistochemical analysis of fibronectin, collagen type III, laminin, and cytokeratin 5 in putrified skin

Betz, P.; Nerlich, A.; Wilske, J.; Tübel, J.; Penning, R. und Eisenmenger, Wolfgang (1993): The immunohistochemical analysis of fibronectin, collagen type III, laminin, and cytokeratin 5 in putrified skin. In: Forensic Science International, Bd. 61, Nr. 1: S. 35-42

Abstract

Fibronectin, collagen type III, laminin, and cytokeratin 5 were visualized in normal skin and in skin showing early or advanced signs of autolytic decomposition to prove whether the immunohistochemical analysis of these antigens can provide useful information for an age-estimation of skin wounds obtained from putrified corpses. In cases with early signs of decomposition (visible course of veins, greenish discoloration) and without microscopic alterations like relaxation of the epidermal cell layers or destruction of the blood vessel structures, the staining pattern was identical to that found in normal, non-putrefied skin. In skin already showing microscopic alteration of the tissue structure, fibronectin and collagen type III could not be localized unambiguously. The distribution of laminin and cytokeratin 5, however, was well preserved. In advanced putrefied skin no reliable staining results could be obtained for fibronectin, collagen type III, and laminin. Even though cytokeratin 5 was still detectable in remnants of decomposition-resistant skin appendages, no information useful for an age-estimation of skin wounds can be obtained due to the autolytic detachment of the epidermal layers.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 0379-0738

Literaturliste

1. H. Thomsen, A. Schulz and S. Bhakdi, Immunhistochemische C 5b-9-Komplement-Komplex Darstellung in Frühstadien der Herzmuskelnekrosen am Paraffinschnitt. Z. Rechtsmed. 103 (1990), pp. 199–206. View Record in Scopus | Cited By in Scopus (0)

2. I. Pedal and J. Hülle, Immunenzymatische Bestimmung des ABO- und Sekretorstatus an paraffin eingebettetem Autopsiematerial. Z. Rechtsmed. 93 (1984), pp. 289–300. View Record in Scopus | Cited By in Scopus (0)

3. I. Ishiyama, M. Mukaida, T. Yoshii and H. Suyama, Histochemical demonstration of methamphetamine by immunocytochemistry. J. Forensic Sci. 32 (1987), pp. 658–672. View Record in Scopus | Cited By in Scopus (13)

4. I. Ishiyama, M. Mukaida, R. Tanabe, M. Kaiho and M. Ueyama, Histochemical demonstration of phenobarbital by immunocytochemistry. J. Forensic Sci. 32 (1987), pp. 1221–1234. View Record in Scopus | Cited By in Scopus (6)

5. M. Nagao, T. Takatai, B. Wu, K. Terazawa, H. Gotouda and H. Akabane, Immunohistochemical localization of paraquat in lung and brain. Med., Sci. Law. 31 (1991), pp. 61–64. View Record in Scopus | Cited By in Scopus (11)

6. G. Fechner, M. Hernandez, T. Bajanowski, M.A. Sepulchre and B. Brinkmann, Immunohistochemical alterations after muscle trauma. Int. J. Leg. Med. 105 (1993), pp. 203–207. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (13)

7. I. Pedal and C. Baedecker, Immunenzymatische Darstellung der Isoantigene A, B und H in fäulnisverändertem Nierengewebe. Z. Rechtsmed. 94 (1985), pp. 9–20. View Record in Scopus | Cited By in Scopus (0)

8. P. Betz, A. Nerlich, J. Wilske, J. Tübel, I. Wiest, R. Penning and W. Eisenmenger, Immunohistochemical localization of fibronectin as a tool for the age determination of human skin wounds. Int. J. Leg. Med. 105 (1992), pp. 21–26. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (31)

9. P. Betz, A. Nerlich, J. Wilske, J. Tübel, I. Wiest, R. Penning and W. Eisenmenger, The time-dependdent rearrangement of the epithelial basement membrane in human skin wounds — immunohistochemical localization of collagens IV and VII. Int. J. Leg. Med. 105 (1992), pp. 93–97. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (10)

10. P. Betz, A. Nerlich, J. Wilske, J. Tübel, I. Wiest, R. Penning and W. Eisenmenger, The temporary pericellular expression of collagen type IV, laminin, and heparan sulfate proteoglycan in myofibroblasts of human skin wounds. Int. J. Leg. Med. 105 (1992), pp. 169–172. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (17)

11. P. Betz, A. Nerlich, J. Wilske, J. Tübel, R. Penning and W. Eisenmenger, The time-dependent reepithelialization of human skin wounds — immunohistochemical localization of keratins 5 and 13. Int. J. Leg. Med. 105 (1993), pp. 229–232. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (3)

12. P. Betz, A. Nerlich, J. Tübel, R. Penning and W. Eisenmenger, The meaning of the immunohistochemical localization of collagens type III and V for the time-estimation of human skin wounds. Int. J. Leg. Med. 105 (1993), pp. 329–332. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (20)

13. W. Eisenmenger, A. Nerlich and G. Gluck, Die Bedeutung des Kollagens bei der Wundaltersbestimmung. Z. Rechtsmed. 100 (1988), pp. 79–100. View Record in Scopus | Cited By in Scopus (19)

14. S.M. Hsu, L. Raine and H. Fanger, A comparative study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radio immunoassay antibodies. Am. J. Clin. Pathol. 75 (1981), pp. 734–739.

15. S. Berg, Die Altersbestimmung von'Hautverletzungen. Z. Rechtsmed. 70 (1972), pp. 121–135. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (3)

16. J. Raekallio, Enzyme Histochemistry of Wound Healing. , Fischer, Stuttgart (1970).