TcellSubC: An Atlas of the Subcellular Proteome of Human T Cells

Joshi, Rubin Narayan; Stadler, Charlotte; Lehmann, Robert; Lehtio, Janne; Tegner, Jesper; Schmidt, Angelika and Vesterlund, Mattias (26. November 2019): TcellSubC: An Atlas of the Subcellular Proteome of Human T Cells. In: Frontiers in Immunology, Vol. 10, 2708

Abstract

We have curated an in-depth subcellular proteomic map of primary human CD4+ T cells, divided into cytosolic, nuclear and membrane fractions generated by an optimized fractionation and HiRIEF-LC-MS/MS workflow for limited amounts of primary cells. The subcellular proteome of T cells was mapped under steady state conditions, as well as upon 15 min and 1 h of T cell receptor (TCR) stimulation, respectively. We quantified the subcellular distribution of 6,572 proteins and identified a subset of 237 potentially translocating proteins, including both well-known examples and novel ones. Microscopic validation confirmed the localization of selected proteins with previously known and unknown localization, respectively. We further provide the data in an easy-to-use web platform to facilitate re-use, as the data can be relevant for basic research as well as for clinical exploitation of T cells as therapeutic targets.

• Item Type: Journal article
• Keywords: Subcellular fractionation; subcellular localization; CD4 T cells; TCR stimulation; protein translocation; mass spectrometry-based proteomics
• Faculties: Medicine
• Subjects: 600 Technology > 610 Medicine and health
• ISSN: 1664-3224
• Language: English