Abstract
We have curated an in-depth subcellular proteomic map of primary human CD4+ T cells, divided into cytosolic, nuclear and membrane fractions generated by an optimized fractionation and HiRIEF-LC-MS/MS workflow for limited amounts of primary cells. The subcellular proteome of T cells was mapped under steady state conditions, as well as upon 15 min and 1 h of T cell receptor (TCR) stimulation, respectively. We quantified the subcellular distribution of 6,572 proteins and identified a subset of 237 potentially translocating proteins, including both well-known examples and novel ones. Microscopic validation confirmed the localization of selected proteins with previously known and unknown localization, respectively. We further provide the data in an easy-to-use web platform to facilitate re-use, as the data can be relevant for basic research as well as for clinical exploitation of T cells as therapeutic targets.
Item Type: | Journal article |
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Keywords: | Subcellular fractionation; subcellular localization; CD4 T cells; TCR stimulation; protein translocation; mass spectrometry-based proteomics |
Faculties: | Medicine |
Subjects: | 600 Technology > 610 Medicine and health |
URN: | urn:nbn:de:bvb:19-epub-78609-2 |
ISSN: | 1664-3224 |
Language: | English |
Item ID: | 78609 |
Date Deposited: | 15. Dec 2021, 14:44 |
Last Modified: | 11. Dec 2023, 16:14 |