A Large Panel of Isogenic APP and PSEN1 Mutant Human iPSC Neurons Reveals Shared Endosomal Abnormalities Mediated by APP beta-CTFs, Not A beta

Kwart, Dylan; Gregg, Andrew; Scheckel, Claudia; Murphy, Elisabeth; Paquet, Dominik; Duffield, Michael; Fak, John; Olsen, Olav; Darnell, Robert und Tessier-Lavigne, Marc (2019): A Large Panel of Isogenic APP and PSEN1 Mutant Human iPSC Neurons Reveals Shared Endosomal Abnormalities Mediated by APP beta-CTFs, Not A beta. In: Neuron, Bd. 104, Nr. 2

Abstract

Familial Alzheimer's disease (fAD) results from mutations in the amyloid precursor protein (APP) and presenilin (PSEN1 and PSEN2) genes. Here we leveraged recent advances in induced pluripotent stem cell (iPSC) and CRISPR/Cas9 genome editing technologies to generate a panel of isogenic knockin human iPSC lines carrying APP and/or PSEN1 mutations. Global transcriptomic and translatomic profiling revealed that fAD mutations have overlapping effects on the expression of AD-related and endocytosis-associated genes. Mutant neurons also increased Rab5+ early endosome size. APP and PSEN1 mutations had discordant effects on Ab production but similar effects on APP beta C-terminal fragments (beta-CTFs), which accumulate in all mutant neurons. Importantly, endosomal dysfunction correlated with accumulation of beta-CTFs, not Ab, and could be rescued by pharmacological modulation of beta-secretase (BACE). These data display the utility of our mutant iPSCs in studying AD-related phenotypes in a non-overexpression human-based system and support mounting evidence that beta-CTF may be critical in AD pathogenesis.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 0896-6273
• Sprache: Englisch