Betz, P.; Tübel, J.; Eisenmenger, Wolfgang; Nerlich, A.; Baretton, G.
Morphological detection of X- and Y-chromosomes in smears and paraffin-embedded tissues using a non-isotopic in situ hybridization technique (NISH).
In: International Journal of Legal Medicine, Vol. 107, No. 2: pp. 108-110
Pharyngeal smears and paraffin-embedded tissue specimens (skeletal muscle, kidney) obtained from 10 male and 10 female individuals were evaluated using non-isotopic in situ hybridization (NISH) with commercial X- and Y-specific biotinylated probes which recognize the pericentromeric regions DXZ1 and DYZ1/DYZ3 of the X- and Y-chromosome, respectively. The results provide evidence that the morphological sex determination of a single cell can be performed by critical application of this staining method leading to one nuclear signal in ldquomalerdquo cells using the Y-specific probe whereas ldquofemalerdquo cells are negative. In situ hybridization of ldquofemalerdquo tissues with an X-specific probe results regularly in 2 signals whereas ldquomalerdquo cells show only one spot in the nucleus.