Glucocorticoid receptors and are modulated sex specifically in human placentas of intrauterine growth restriction (IUGR)

Hutter, Stefan; Hepp, Paula; Hofmann, Simone; Kuhn, Christina; Messner, Julia; Andergassen, Ulrich; Mayr, Doris; Emilia Solano, Maria; Obermeier, Viola; Mahner, Sven; Arck, Petra und Jeschke, Udo (2019): Glucocorticoid receptors and are modulated sex specifically in human placentas of intrauterine growth restriction (IUGR). In: Archives of Gynecology and Obstetrics, Bd. 300, Nr. 2: S. 323-335

Abstract

PurposeThe objective of this study was to analyze the expression of the glucocorticoid receptor (GR) subtypes GR and GR in placentas affected by intrauterine growth restriction (IUGR).MethodsWe analyzed the sex-specific placental expression of GR and GR in 23 IUGR and 40 control placentas using immunohistochemistry and immunofluorescence. The GR gene, also known as nuclear receptor subfamily 3 group C member 1 (NR3C1), mRNA production in trophoblast-like cell line BeWo after stimulation with prednisolone was analyzed using quantitative polymerase chain reaction (qPCR) and on the protein level using western blot analysis.ResultsGR subtypes showed a sex-specific upregulation in placentas from IUGR compared to control placentas. An increased expression of GR was detectable in female placental tissue, whereas GR was increased in males.ConclusionOur data support previous findings suggesting that the glucocorticoid metabolism plays a role in the pathophysiology of IUGR. Furthermore, the data suggest that the underlying molecular mechanisms differ between male and female cases.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 0932-0067
• Sprache: Englisch