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Gazquez, Antonio; Prieto-Sanchez, Maria T.; Blanco-Carnero, Jose E.; van Harskamp, Dewi; Perazzolo, Simone; Oosterink, J. Efraim; Demmelmair, Hans; Schierbeek, Henk; Sengers, Bram G.; Lewis, Rohan M.; van Goudoever, Johannes B.; Koletzko, Berthold; Larque, Elvira (2019): In vivo kinetic study of materno-fetal fatty acid transfer in obese and normal weight pregnant women. In: Journal of Physiology-London, Vol. 597, No. 19: pp. 4959-4973
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We analyse for the first time the in vivo materno-fetal kinetic transfer of fatty acids (FA) labelled with stable isotopes in control and obese (OB) pregnant women. Labelled FA with a similar metabolism (stearic acid: C-13-SA;palmitic acid: C-13-PA;oleic acid: C-13-OA) were orally administered at -4 h, -8 h and -12 h, respectively prior to elective caesarean section to 10 pregnant women with a body mass index >30 (OB) and 10 with a body mass index in the range 20-25 (NW). Placenta, venous and arterial cord blood were collected obtaining a wide range of FA enrichments. A combined experimental and computational modelling analysis was applied. FA fractional synthesis rate (FSR) in placenta was 11-12% h(-1). No differences were observed between NW and normo-lipidemic OB. It was not possible to estimate FA FSR in cord blood with this oral bolus dose approach. Computational modelling demonstrated a good fit to the data when all maternal plasma lipid classes were included but not with modelling based only on the non-esterified FA fraction. The estimated materno-fetal C-13-FA transfer was similar to 1%. In conclusion, our approach using multiple C-13-FA tracers allowed us to estimated FSR in placental/maternal plasma but not in fetal/maternal compartments. Computational modelling showed a consistent time course of placental C-13-FA transfer and predicted total fetal FA accumulation during the experiment. We conclude that, in addition to non-esterified FA fraction in the maternal circulation, maternal plasma very low-density lipoprotein and other lipoproteins are important contributors to placental FA transfer to the fetus.