MCC950 blocks enhanced interleukin-1 beta production in patients with NLRP3 low penetrance variants

Schuh, E.; Gross, C. J.; Wagner, D.; Schlueter, M.; Gross, O. und Kuempfel, T. (2019): MCC950 blocks enhanced interleukin-1 beta production in patients with NLRP3 low penetrance variants. In: Clinical Immunology, Bd. 203: S. 45-52

Abstract

Objective: To determine the role of the NLRP3 inflammasome by using the selective NLRP3 inhibitor MCC950 in patients with NLRP3 low penetrance variants and clinical symptoms suggestive for an autoinflammatory syndrome including central nervous system (CNS) involvement. Methods: Nineteen symptomatic patients with low penetrance NLRP3 variants (Q703K n = 17, V198M n = 2) recruited between 2011 and 2017 were included in this monocentric study. A functional inflammasome activation assay was performed in patients in comparison to healthy controls (HC), including the determination of interleukin-lbeta (IL-1 beta), interleukin-6 (IL-6) and tumor-necrosis factor alpha (TNF-alpha) secretion in the presence of the NLRP3 selective small-molecule inhibitor MCC950. Detailed clinical features were assessed and anti-IL-1 treatment response was determined. Results: Peripheral blood mononuclear cells (PBMC) from patients with low penetrance NLRP3 variants displayed enhanced IL-1 beta levels following inflammasome activation compared to HC. Furthermore, IL-1 beta release was NLRP3-dependent as it was blocked by MCC950. The production of IL-6 and TNF-alpha was also increased in patients with low penetrance NLRP3 variants. Clinically, they presented with a heterogenous spectrum of neurological manifestations, while cranial nerve inflammation was the most common feature. Overall inflammasome activation did not correlate with disease severity. Eight of ten treated patients responded to anti IL-1 treatment, however a complete response was only documented in four patients. Conclusion: PBMC of several patients with NLRP3 low penetrance variants and CNS manifestation showed increased NLRP3-specific IL-1 beta release upon stimulation and elevated NLRP3-independent IL-6 and TNF-alpha levels as those were not suppressed by MCC950. Our data suggest that beside the possible causal involvement of the NLRP3 inflammasome additional, yet unidentified genetic or environmental factors may contribute to the multi organ inflammation in our patients and explain the partial response to IL-1 targeting therapies.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 1521-6616
• Sprache: Englisch