Influence of static forces on the expression of selected parameters of inflammation in periodontal ligament cells and alveolar bone cells in a co-culture in vitro model

Shi, Jianwei; Baumert, Uwe; Folwaczny, Matthias und Wichelhaus, Andrea (2019): Influence of static forces on the expression of selected parameters of inflammation in periodontal ligament cells and alveolar bone cells in a co-culture in vitro model. In: Clinical Oral Investigations, Bd. 23, Nr. 6: S. 2617-2628

Abstract

ObjectiveAim of this study was to investigate the impact of human PDL-derived fibroblasts (HPDF) and human alveolar bone-derived osteoblasts (HABO) co-culture on the expression of cytokines involved in tissue remodeling using an in vitro compressive force (CF) model.Materials and methodsStatic compressive force (CF) of 47.4g/cm(2) was applied on mono- and co-cultured HPDFs and HABOs for 1, 2, or 4h at 30 degrees C. TNFA, PTGS2, and IL6 gene expressions were determined by quantitative real-time polymerase chain reaction. TNF, PGE(2), and IL6 concentrations were measured using enzyme-linked immunosorbent assay.ResultsIn mono-culture, TNFA, PTGS2, and IL6 gene expressions were upregulated under CF as compared to controls for each time period in both cell types. PGE(2) increased at 1 and 2h in both cell types, and IL6 increased only at 2 and 4h in HPDFs. Co-culture alleviated the force-induced increase of the expression of TNFA, PTGS2, IL6, PGE2, and IL6 in HPDFs at any time point. In HABOs, co-cultivation decreased the expression of PGE(2) after 1h and 4h, and that of IL6 after 1h compared to mono-culture.ConclusionsCF application on co-cultures of HPDFs and HABOs causes significant changes of TNFA, PTGS2, and IL6 gene expressions and PGE(2) and IL6 production in comparison to mono-culture indicating intercellular communication.Clinical relevanceMechanical stimulation of HPDFs and HABOs in co-culture induces a different gene expression pattern than stimulation of individual cell types alone. Co-culture might therefore be a relevant method to elucidate periodontal regeneration during orthodontic therapy.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 1432-6981
• Sprache: Englisch