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Beck, Felicitas; Hartmann, Eliza S.; Köhler, Miriam I.; Redeker, Julia I.; Schluessel, Sabine; Schmitt, Baerbel; Fottner, Andreas; Unger, Marina; van Griensven, Martijn; Michael, Jan; Summer, Burkhard; Kunzelmann, Karl-Heinz; Beutner, Rene; Scharnweber, Dieter; Kostenuik, Paul J.; Mayer-Wagner, Susanne (2019): Immobilization of Denosumab on Titanium Affects Osteoclastogenesis of Human Peripheral Blood Monocytes. In: International Journal of Molecular Sciences, Vol. 20, No. 5, 1002
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Abstract

Immobilization of proteins has been examined to improve implant surfaces. In this study, titanium surfaces were modified with nanofunctionalized denosumab (cDMAB), a human monoclonal anti-RANKL IgG. Noncoding DNA oligonucleotides (ODN) served as linker molecules between titanium and DMAB. Binding and release experiments demonstrated a high binding capacity of cDMAB and continuous release. Human peripheral mononuclear blood cells (PBMCs) were cultured in the presence of RANKL/MCSF for 28 days and differentiated into osteoclasts. Adding soluble DMAB to the medium inhibited osteoclast differentiation. On nanofunctionalized titanium specimens, the osteoclast-specific TRAP5b protein was monitored and showed a significantly decreased amount on cDMAB-titanium in PBMCs + RANKL/MCSF. PBMCs on cDMAB-titanium also changed SEM cell morphology. In conclusion, the results indicate that cDMAB reduces osteoclast formation and has the potential to reduce osteoclastogenesis on titanium surfaces.