Increased plasticity of non-classic Th1 cells toward the Th17 phenotype

Leipe, Jan; Pirronello, Fausto; Klose, Antonia; Schulze-Koops, Hendrik and Skapenko, Alla (2019): Increased plasticity of non-classic Th1 cells toward the Th17 phenotype. In: Modern Rheumatology

Abstract

Objectives: To analyze occurrence and plasticity of two recently described distinct subtypes of Th1 cells named classic (CD161?/CCR6?) and non-classic (CD161+/CCR6+) Th1 cells in early rheumatoid arthritis (RA) patients and healthy controls (HCs). Methods: Frequencies of in vivo-generated Th1 cell populations were assessed after cytokine secretion assay for IFN?/IL-17 and surface staining for CD161/CCR6. Viable Th1 cells (IFN?+IL-17?) were sorted into classic Th1 (CD161-CCR6?) and non-classic Th1 (CD161+CCR6+) cells, trans-differentiated under different Th cell-inducing conditions, and assessed for plastic changes by analyzing the Th cell-associated cytokine and transcription factor profiles. Results: Ex vivo frequencies of classic (CD161?CCR6?) and non-classic (CD161+CCR6+) Th1 cells as well as related Th1 cell subpopulations CD161+CCR6? and CD161?/CCR6+ did not differ significantly between RA and HCs. However, trans-differentiation of ex vivo non-classic (CD161+CCR6+) and CD161?/CCR6+ Th1 cells resulted in a substantial shift toward Th17 and Th1/Th17 phenotypes, particularly under Th17-inducing conditions. In contrast, classic (CD161?/CCR6?) and CD161+CCR6? Th1 cells showed higher plasticity towards IL-4-producing cells, most of them shifting to a Th1/Th2 phenotype. Conclusion: Whereas non-classic (CD161+/CCR6+) and CD161-CCR6+ Th1 cells demonstrated an increased plasticity towards IL-17- phenotypes, classic Th1 and CD161+CCR6? Th1 cells showed more plasticity towards IL-4-producing phenotypes.

• Item Type: Journal article
• Faculties: Medicine
• Subjects: 600 Technology > 610 Medicine and health
• ISSN: 1439-7595
• Language: English