Abstract
We present a two-color two-photon stimulated emission depletion microscopy technique (2C2P-STED) that correlates a confocal image with a super-resolved image employing the inherent self-referencing mechanism of nonlinear excitation. The novel approach overcomes the substantial challenge posed by two different imaging modalities in laser-scanning fluorescence microscopy for colocalization on the nanometer scale. Demonstrating the principle of 2C2P-STED, we show for the first time super-resolved images of the gram-positive bacteria Streptococcus pneumoniae TIGR4 pilus type- 1 . A signal-to-noise ratio (SINIR) greater than 10 was achieved in 2C2P excitation mode and approximately 70 nm details were resolved in 2P-STED. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Item Type: | Journal article |
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Faculties: | Physics |
Subjects: | 500 Science > 530 Physics |
ISSN: | 2156-7085 |
Language: | English |
Item ID: | 82674 |
Date Deposited: | 15. Dec 2021, 15:02 |
Last Modified: | 08. Nov 2023, 18:12 |