TuPPL: Tub-tag mediated C-terminal protein-protein-ligation using complementary click-chemistry handles

Stengl, Andreas; Gerlach, Marcus; Kasper, Marc-Andre; Hackenberger, Christian P. R.; Leonhardt, Heinrich; Schumacher, Dominik und Helma, Jonas (2019): TuPPL: Tub-tag mediated C-terminal protein-protein-ligation using complementary click-chemistry handles. In: Organic & Biomolecular Chemistry, Bd. 17, Nr. 20: S. 4964-4969

Abstract

We introduce a chemoenzymatic strategy for straightforward in vitro generation of C- terminally linked fusion proteins. Tubulin tyrosine ligase is used for the incorporation of complementary click chemistry handles facilitating subsequent formation of functional bispecific antibody- fragments. This simple strategy may serve as central conjugation hub for a modular protein ligation platform.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Biologie > Department Biologie II
• Themengebiete: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
• ISSN: 1477-0520
• Sprache: Englisch