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Stengl, Andreas; Gerlach, Marcus; Kasper, Marc-Andre; Hackenberger, Christian P. R.; Leonhardt, Heinrich; Schumacher, Dominik; Helma, Jonas (2019): TuPPL: Tub-tag mediated C-terminal protein-protein-ligation using complementary click-chemistry handles. In: Organic & Biomolecular Chemistry, Vol. 17, No. 20: pp. 4964-4969
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We introduce a chemoenzymatic strategy for straightforward in vitro generation of C- terminally linked fusion proteins. Tubulin tyrosine ligase is used for the incorporation of complementary click chemistry handles facilitating subsequent formation of functional bispecific antibody- fragments. This simple strategy may serve as central conjugation hub for a modular protein ligation platform.