TuPPL: Tub-tag mediated C-terminal protein-protein-ligation using complementary click-chemistry handles

Stengl, Andreas; Gerlach, Marcus; Kasper, Marc-Andre; Hackenberger, Christian P. R.; Leonhardt, Heinrich; Schumacher, Dominik and Helma, Jonas (2019): TuPPL: Tub-tag mediated C-terminal protein-protein-ligation using complementary click-chemistry handles. In: Organic & Biomolecular Chemistry, Vol. 17, No. 20: pp. 4964-4969

Abstract

We introduce a chemoenzymatic strategy for straightforward in vitro generation of C- terminally linked fusion proteins. Tubulin tyrosine ligase is used for the incorporation of complementary click chemistry handles facilitating subsequent formation of functional bispecific antibody- fragments. This simple strategy may serve as central conjugation hub for a modular protein ligation platform.

• Item Type: Journal article
• Faculties: Biology > Department Biology II
• Subjects: 500 Science > 570 Life sciences; biology
• ISSN: 1477-0520
• Language: English