Abstract
Only two drugs are currently available for the treatment of Chagas disease and their effectiveness are unsatisfactory. Photorhabdus luminescens and Xenorhabdus nematophila, two enteric bacteria highly pathogenic to a broad range of insects, have been studied as potential source for bioactive metabolites against protozoa causing neglected tropical diseases. Therefore, we tested the in vitro anti-Trypanosoma cruzi activity of secreted metabolites from these bacteria. The conditioned medium of X. nematophila and P. luminescens showed significant parasiticidal activity in a concentration-dependent manner (IC50 XN = 0.34 mg/mL, IC50 PL = 1.0 mg/mL). The parasiticidal compound was identified as a small molecule stable to heating and pH changes ranging from 2 to 12. Moreover, anti-Trypanosoma molecules secreted by both bacteria stimulate the trypanocidal activity of macrophages by a mechanism independent of nitric oxide. Summarizing, our studies reveal that P. luminescens and X. nematophila are potential sources of putative novel drugs against Chagas disease.
Item Type: | Journal article |
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Faculties: | Biology > Department Biology II |
Subjects: | 500 Science > 570 Life sciences; biology |
ISSN: | 0014-4894 |
Language: | English |
Item ID: | 83941 |
Date Deposited: | 15. Dec 2021, 15:09 |
Last Modified: | 15. Dec 2021, 15:09 |