Single-Molecule Biophysical Techniques to Study Actomyosin Force Transduction

Takagi, Yasuharu; Hundt, Nikolas und Fineberg, Adam (2020): Single-Molecule Biophysical Techniques to Study Actomyosin Force Transduction. In: Coluccio, Lynne M. (Hrsg.): Myosins. A Superfamily of Molecular Motors. 1239, Cham: Springer. S. 85-126

Abstract

Inside the cellular environment, molecular motors can work in concert to conduct a variety of important physiological functions and processes that are vital for the survival of a cell. However, in order to decipher the mechanism of how these molecular motors work, single-molecule microscopy techniques have been popular methods to understand the molecular basis of the emerging ensemble behavior of these motor proteins.In this chapter, we discuss various single-molecule biophysical imaging techniques that have been used to expose the mechanics and kinetics of myosins. The chapter should be taken as a general overview and introductory guide to the many existing techniques; however, since other chapters will discuss some of these techniques more thoroughly, the readership should refer to those chapters for further details and discussions. In particular, we will focus on scattering-based single-molecule microscopy methods, some of which have become more popular in the recent years and around which the work in our laboratories has been centered.

• Dokumententyp: Buchbeitrag
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISBN: 978-3-030-38061-8 (print) ; 978-3-030-38062-5 (Online)
• Ort: Cham
• Sprache: Englisch