Subcellular Compartmentation of Metabolites Involved in Cold Acclimation

Hoermiller, Imke I.; Nägele, Thomas and Heyer, Arnd G. (2020): Subcellular Compartmentation of Metabolites Involved in Cold Acclimation. In: Hincha, Dirk K. and Zuther, Ellen (eds.) : Plant Cold Acclimation : Methods and Protocols. Methods in molecular biology, Vol. 2156. Cham: Springer. pp. 269-275

Abstract

Plant cells are heavily compartmentalized, and metabolite concentrations in the various compartments differ significantly. Thus, determination of metabolite abundance in whole-cell extracts may be misleading, when the role of a compound in plant freezing tolerance shall be evaluated. Here, we describe a method for the separation of the largest compartments of plant cells, the vacuole, plastid, and cytosol. With more elaborate analysis, this method can be expanded to also resolve mitochondria and other compartments.

• Item Type: Book Section
• Faculties: Biology > Department Biology I
• Subjects: 500 Science > 570 Life sciences; biology
• ISBN: 978-1-0716-0659-9 ; 978-1-0716-0662-9 ; 978-1-0716-0660-5
• Place of Publication: Cham
• Language: English