Effect of static compressive force on in vitro cultured PDL fibroblasts: monitoring of viability and gene expression over 6 days

Janjic Rankovic, Mila; Docheva, Denitsa; Wichelhaus, Andrea und Baumert, Uwe (2020): Effect of static compressive force on in vitro cultured PDL fibroblasts: monitoring of viability and gene expression over 6 days. In: Clinical Oral Investigations, Bd. 24, Nr. 7: S. 2497-2511

Abstract

Objectives: The aim was to investigate the impact of static compressive force (CF) application on human PDL-derived fibroblasts (HPDF) in vitro for up to 6 days on the expression of specific genes and to monitor cell growth and cell viability. Materials and methods: CF of 2 g/cm(2)was applied on HPDFs for 1-6 days. On each day, gene expression (cFOS, HB-GAM, COX2,IL6,TNF alpha,RUNX2, andP2RX2) and secretion (TNF alpha, PGE(2)) were determined by RT-qPCR and ELISA, respectively. Cell growth and cell viability were monitored daily. Results: In comparison with controls, significant upregulation ofcFOSin compressed HPDFs was observed.HB-GAMshowed no changes in expression, except on day 5 (P < 0.001).IL6expression was significantly upregulated from day 2-5, reaching the maximum on day 3 (P < 0.001).TNF alpha expression was upregulated on all but day 2.COX2showed upregulation, reaching the plateau from day 3 (P < 0.001) until day 4 (P < 0.001), and returning to the initial state till day 6.P2RX7was downregulated on days 2 and 4 to 6 (P < 0.001).RUNX2was downregulated on days 2 and 5 (bothP < 0.001). Cells in both groups were proliferating, and no negative effect on cell viability was observed. Conclusion Results suggest high molecular activity up to 6 days, therefore introducing further need for in vitro studies with a longer duration that would explain other genes and metabolites involved in orthodontic tooth movement (OTM).

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 1432-6981
• Sprache: Englisch