Molecular imaging of cardiac CXCR4 expression in a mouse model of acute myocardial infarction using a novel Ga-68-mCXCL12 PET tracer

Zacherl, Mathias Johannes; Todica, Andrei; Wngler, Carmen; Schirrmacher, Ralf; Hajebrahimi, Mohammad Ali; Pircher, Joachim; Li, Xiang; Lindner, Simon; Brendel, Matthias; Bartenstein, Peter; Massberg, Steffen; Brunner, Stefan; Lehner, Sebastian; Hacker, Marcus und Huber, Bruno C. (2020): Molecular imaging of cardiac CXCR4 expression in a mouse model of acute myocardial infarction using a novel Ga-68-mCXCL12 PET tracer. In: Journal of Nuclear Cardiology

Abstract

Background: The chemokine receptor CXCR4 and its ligand CXCL12 have been shown to be a possible imaging and therapeutic target after myocardial infarction (MI). The murine-based and mouse-specific(68)Ga-mCXCL12 PET tracer could be suitable for serial in vivo quantification of cardiac CXCR4 expression in a murine model of MI. Methods and Results At days 1-6 after MI, mice were intravenously injected with(68)Ga-mCXCL12. Autoradiography was performed and the infarct-to-remote ratio (I/R) was determined. In vivo PET imaging with(68)Ga-mCXCL12 was conducted on days 1-6 after MI and the percentage of the injected dose (%ID/g) of the tracer uptake in the infarct area was calculated.F-18-FDG-PET was performed for anatomical landmarking. Ex vivo autoradiography identified CXCR4 upregulation in the infarct region with an increasingI/Rafter 12 hours (1.4 +/- 0.3), showing a significant increase until day 2 (4.5 +/- 0.6), followed by a plateau phase (day 4) and decrease after 10 days (1.3 +/- 1.0). In vivo PET imaging identified similar CXCR4 upregulation in the infarct region which peaked around day 3 post MI (9.7 +/- 5.0 %ID/g) and then subsequently decreased by day 6 (2.8 +/- 1.0 %ID/g). Conclusion Noninvasive molecular imaging of cardiac CXCR4 expression using a novel, murine-based, and specific(68)Ga-mCXCL12 tracer is feasible both ex vivo and in vivo.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 1071-3581
• Sprache: Englisch