A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function

Perry, Christopher J.; Warren, Eleanor C.; Damstra-Oddy, Joseph L.; Storey, Claire; Francione, Lisa M.; Annesley, Sarah J.; Fisher, Paul R.; Müller-Taubenberger, Annette und Williams, Robin S. B. (2020): A Dictyostelium discoideum mitochondrial fluorescent tagging vector that does not affect respiratory function. In: Biochemistry and Biophysics Reports, Bd. 22, 100751

Abstract

Visualizing mitochondria in living Dictyostelium discoideum cells using fluorescent dyes is often problematic due to variability in staining, metabolism of the dyes, and unknown potential effects of the dyes on mitochondrial function. We show that fluorescent labelling of mitochondria, using an N-terminal mitochondrial localization sequence derived from the D. discoideum protein GcvH1 (glycine cleavage system H1) attached to a red fluorescent protein enables clear mitochondrial imaging. We also show that this labelling has no effect upon mitochondria load or respiratory function.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 2405-5808
• Sprache: Englisch