
Abstract
We describe the identification and submitochondrial localization of four protein kinases and of their target proteins in derepressed yeast mitochondria. The activity of one of the kinases depends on the presence of cyclic AMP (cAMP). It is soluble and localized in the mitochondrial intermembrane space. Its natural target is a polypeptide of 40 kDa molecular mass, which is bound to the inner membrane. Besides this natural target this kinase phosphorylates acidic heterologous proteins, like casein, with high efficiency. The other protein kinases identified so far are cAMP-independent. At least one is localized in the matrix having its natural substrates (49 and 24 kDa) in the same compartment. Two others are firmly bound to the inner membrane phosphorylating target proteins in the inner membrane (52·5 kDa) and in the intermembrane space (17·5 kDa), respectively.
Item Type: | Journal article |
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Keywords: | Saccharomyces cerevisiae, mitochondria, cAMP-dependent protein kinase, submitochondrial localization, topology |
Faculties: | Biology > Department Biology I |
Subjects: | 500 Science > 570 Life sciences; biology |
URN: | urn:nbn:de:bvb:19-epub-8558-9 |
ISSN: | 1097-0061 |
Signature: | BSB:4 Z 85.175-3 |
Item ID: | 8558 |
Date Deposited: | 25. Mar 2009, 15:00 |
Last Modified: | 04. Nov 2020, 12:51 |