Binding patterns and functional properties of human antibodies to AQP4 and MOG on murine optic nerve and retina

Faissner, Simon; Graz, Florian; Reinehr, Sabrina; Petrikowski, Laura; Haupeltshofer, Steffen; Ceylan, Ulas; Stute, Gesa; Winklmeier, Stephan; Pache, Florence; Paul, Friedemann; Ruprecht, Klemens; Meinl, Edgar; Dick, H. Burkhard; Gold, Ralf; Kleiter, Ingo and Joachim, Stephanie C. (2020): Binding patterns and functional properties of human antibodies to AQP4 and MOG on murine optic nerve and retina. In: Journal of Neuroimmunology, Vol. 342, 577194

Abstract

Neuromyelitis optica spectrum disorder (NMOSD) is an autoimmune-inflammatory CNS disease affecting spinal cord and optic nerves, mediated by autoantibodies against aquaporin-4 (AQP4) and myelin-oligodendrocyteglycoprotein (MOG). Effects of those immunoglobulins (Ig) on retina and optic nerve are incompletely understood. We investigated AQP4-IgG and MOG-IgG sera on retina and optic nerve ex vivo and in 2D2 mice, which harbor a transgenic MOG-specific T-cell receptor. Some sera reacted with murine retina and optic nerve showing distinct binding patterns, suggesting different epitopes being targeted in both subgroups. Transfer of total IgG from a MOG-IgG positive patient to 2D2 mice did neither enhance disability nor induce functional or histological alterations in the retina.

• Item Type: Journal article
• Faculties: Medicine
• Subjects: 600 Technology > 610 Medicine and health
• ISSN: 0165-5728
• Language: English