Logo Logo
Hilfe
Hilfe
Switch Language to English

Murawska, Magdalena; Schauer, Tamas; Matsuda, Atsushi; Wilson, Marcus D.; Pysik, Thomas; Wojcik, Felix; Muir, Tom W.; Hiraoka, Yasushi; Straub, Tobias und Ladurner, Andreas G. (2020): The Chaperone FACT and Histone H2B Ubiquitination Maintain S. pombe Genome Architecture through Genic and Subtelomeric Functions. In: Molecular Cell, Bd. 77, Nr. 3: S. 501-513

Volltext auf 'Open Access LMU' nicht verfügbar.

Abstract

The histone chaperone FACT and histone H2B ubiquitination (H2Bub) facilitate RNA polymerase II (Pol II) passage through chromatin, yet it is not clear how they cooperate mechanistically. We used genomics, genetic, biochemical, and microscopic approaches to dissect their interplay in Schizosaccharomyces pombe. We show that FACT and H2Bub globally repress antisense transcripts near the 50 end of genes and inside gene bodies, respectively. The accumulation of these transcripts is accompanied by changes at genic nucleosomes and Pol II redistribution. H2Bub is required for FACT activity in genic regions. In the H2Bub mutant, FACT binding to chromatin is altered and its association with histones is stabilized, which leads to the reduction of genic nucleosomes. Interestingly, FACT depletion globally restores nucleosomes in the H2Bub mutant. Moreover, in the absence of Pob3, the FACT Spt16 subunit controls the 30 end of genes. Furthermore, FACT maintains nucleosomes in subtelomeric regions, which is crucial for their compaction.

Dokument bearbeiten Dokument bearbeiten