Patient-derived malignant pleural mesothelioma cell cultures: a tool to advance biomarker-driven treatments

Kanellakis, Nikolaos; Asciak, Rachelle; Hamid, Megat Abd; Yao, Xuan; McCole, Mark; McGowan, Simon; Seraia, Elena; Hatch, Stephanie; Hallifax, Rob J.; Mercer, Rachel M.; Bedawi, Eihab O.; Jones, Stephanie; Verrill, Clare; Dobson, Melissa; George, Vineeth; Stathopoulos, Georgios T.; Peng, Yanchun; Ebner, Daniel; Dong, Tao; Rahman, Najib M. und Psallidas, Ioannis (2020): Patient-derived malignant pleural mesothelioma cell cultures: a tool to advance biomarker-driven treatments. In: Thorax, Bd. 75, Nr. 11: S. 1004-1008

Abstract

Malignant pleural mesothelioma (MPM) is an aggressive cancer, associated with poor prognosis. We assessed the feasibility of patient-derived cell cultures to serve as an ex vivo model of MPM. Patient-derived MPM cell cultures (n=16) exhibited stemness features and reflected intratumour and interpatient heterogeneity. A subset of the cells were subjected to high-throughput drug screening and coculture assays with cancer-specific cytotoxic T cells and showed diverse responses. Some of the biphasic MPM cells were capable of processing and presenting the neoantigen SSX-2 endogenously. In conclusion, patient-derived MPM cell cultures are a promising and faithful ex vivo model of MPM.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 0040-6376
• Allianz-/Nationallizenz: Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
• Sprache: Englisch