Only Hyperuricemia with Crystalluria, but not Asymptomatic Hyperuricemia, Drives Progression of Chronic Kidney Disease

Sellmayr, Markus; Petzsche, Moritz Roman Hernandez; Ma, Qiuyue; Krueger, Nils; Liapis, Helen; Brink, Andreas; Lenz, Barbara; Angelotti, Maria Lucia; Gnemmi, Viviane; Kuppe, Christoph; Kim, Hyojin; Bindels, Eric Monique Johannes; Tajti, Ferenc; Saez-Rodriguez, Julio; Lech, Maciej; Kramann, Rafael; Romagnani, Paola; Anders, Hans-Joachim und Steiger, Stefanie (2020): Only Hyperuricemia with Crystalluria, but not Asymptomatic Hyperuricemia, Drives Progression of Chronic Kidney Disease. In: Journal of the American Society of Nephrology, Bd. 31, Nr. 12: S. 2773-2792

Abstract

Background: The roles of asymptomatic hyperuricemia or uric acid (UA) crystals in CKD progression are unknown. Hypotheses to explain links between UA deposition and progression of CKD include that (1) asymptomatic hyperuricemia does not promote CKD progression unless UA crystallizes in the kidney;(2) UA crystal granulomas may form due to pre-existing CKD;and (3) proinflammatory granuloma-related M1-like macrophages may drive UA crystal-induced CKD progression. Methods MALDI-FTICR mass spectrometry, immunohistochemistry, 3D confocal microscopy, and flow cytometry were used to characterize a novel mouse model of hyperuricemia and chronic UA crystal nephropathy with granulomatous nephritis. Interventional studies probed the role of crystal-induced inflammation and macrophages in the pathology of progressive CKD. Results Asymptomatic hyperuricemia alone did not cause CKDor drive the progression of aristolochic acid I-induced CKD. Only hyperuricemia with UA crystalluria due to urinary acidification caused tubular obstruction, inflammation, and interstitial fibrosis. UA crystal granulomas surrounded by proinflammatory M1-likemacrophages developed late in this process of chronic UA crystal nephropathy and contributed to the progression of pre-existing CKD. SuppressingM1-like macrophages with adenosine attenuated granulomatous nephritis and the progressive decline inGFR. In contrast, inhibiting the JAK/STAT inflammatory pathway with tofacitinib was not renoprotective. Conclusions: Asymptomatic hyperuricemia does not affect CKD progression unless UA crystallizes in the kidney. UA crystal granulomas develop late in chronic UA crystal nephropathy and contribute to CKD progression because UA crystals trigger M1-like macrophage-related interstitial inflammation and fibrosis. Targeting proinflammatory macrophages, but not JAK/STATsignaling, can attenuategranulomatous interstitial nephritis.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• ISSN: 1046-6673
• Sprache: Englisch