Microbiome Analysis from Paired Mucosal and Fecal Samples of a Colorectal Cancer Biobank

Wirth, Ulrich; Garzetti, Debora; Jochum, Lara M.; Spriewald, Stefanie; Kuhn, Florian; Ilmer, Matthias; Lee, Serene M. L.; Niess, Hanno; Bazhin, Alexandr V.; Andrassy, Joachim; Werner, Jens; Stecher, Barbara und Schiergens, Tobias S. (2020): Microbiome Analysis from Paired Mucosal and Fecal Samples of a Colorectal Cancer Biobank. In: Cancers, Bd. 12, Nr. 12, 3702

Abstract

Simple Summary The role of gut microbiota in colorectal cancer is subject to extensive research. The aim of this study was to assess the feasibility of DNA extraction and microbiome profiling of samples from different sample sites, tissue sites and storage duration of a colorectal cancer biobank. Mucosa samples, mucosal scrapings and feces as well as different tissue sites (tumor, normal mucosa) were analyzed. Microbiome analysis could be successfully performed in most of the samples (overall 93.3%) with sufficient numbers of high-quality reads. There were no differences between sample sites, while in some measures, significant differences were found between tumor and normal mucosa. Samples stored for up to eight years were used and storage conditions had no significant influence on the results. Microbiome analysis can be carried out successfully in fecal, normal mucosal and tumor samples stored long term in a colorectal cancer biobank, hence large retrospective microbiome association studies are feasible. The role of gut microbiota in colorectal cancer is subject to extensive research. Before usage of biorepositories for microbiome studies, it is crucial to evaluate technical feasibility of microbiome profiling from various biospecimens. The aim of this study was to assess the feasibility of DNA-extraction and microbiome profiling of samples from different sample sites, tissue sites and storage duration of a colorectal cancer biobank. Mucosa samples, mucosal scrapings and feces as well as different tissue sites (tumor, normal mucosa) were analyzed. 16S rRNA gene-based microbiome profiling with taxonomic assignment was performed on the Illumina MiSeq (Illumina, San Diego, USA) platform from stored snap frozen samples. For statistical analysis, alpha- and beta-diversity measures, PCoA, permutational multivariate analysis of variance and graphical representation were performed. Microbiome analysis could be successfully performed in most of the samples (overall 93.3%) with sufficient numbers of high-quality reads. There were no differences between sample sites, while in some measures significant differences were found between tumor and normal mucosa (alpha-diversity, Shannon/Simpson Indices p = 0.028/0.027, respectively). Samples stored for up to eight years were used and storage conditions had no significant influence on the results. Tumor and tissue samples of a biobank stored long term can be successfully used for microbiome analysis. As large sample sizes are needed for association studies to evaluate microbial impact on tumorigenesis or progression of colorectal cancer, an already established biorepository may be a useful alternative to prospective clinical studies.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Medizin
• Themengebiete: 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
• Sprache: Englisch