Identification of an Intermediate Step in Foamy Virus Fusion

Dupont, Aurelie; Gluck, Ivo M.; Ponti, Dorothee; Stirnnagel, Kristin; Hutter, Sylvia; Perrotton, Florian; Stanke, Nicole; Richter, Stefanie; Lindemann, Dirk und Lamb, Don C. (2020): Identification of an Intermediate Step in Foamy Virus Fusion. In: Viruses-Basel, Bd. 12, Nr. 12, 1472

Abstract

Viral glycoprotein-mediated membrane fusion is an essential step for productive infection of host cells by enveloped viruses;however, due to its rarity and challenges in detection, little is known about the details of fusion events at the single particle level. Here, we have developed dual-color foamy viruses (FVs) composed of eGFP-tagged prototype FV (PFV) Gag and mCherry-tagged Env of either PFV or macaque simian FV (SFVmac) origin that have been optimized for detection of the fusion process. Using our recently developed tracking imaging correlation (TrIC) analysis, we were able to detect the fusion process for both PFV and SFVmac Env containing virions. PFV Env-mediated fusion was observed both at the plasma membrane as well as from endosomes, whereas SFVmac Env-mediated fusion was only observed from endosomes. PFV Env-mediated fusion was observed to happen more often and more rapidly than as for SFVmac Env. Strikingly, using the TrIC method, we detected a novel intermediate state where the envelope and capsids are still tethered but separated by up to 400 nm before final separation of Env and Gag occurred.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Chemie und Pharmazie > Department Chemie
• Fakultätsübergreifende Einrichtungen: Center for NanoScience (CENS)
• Themengebiete: 500 Naturwissenschaften und Mathematik > 540 Chemie ; 500 Naturwissenschaften und Mathematik > 500 Naturwissenschaften
• Sprache: Englisch