Photolipid Bilayer Permeability is Controlled by Transient Pore Formation

Pritzl, Stefanie D.; Urban, Patrick; Prasselsperger, Alexander; Konrad, David B.; Frank, James A.; Trauner, Dirk und Lohmueller, Theobald (2020): Photolipid Bilayer Permeability is Controlled by Transient Pore Formation. In: Langmuir, Bd. 36, Nr. 45: S. 13509-13515

Abstract

Controlling the release or uptake of (bio-) molecules and drugs from liposomes is critically important for a range of applications in bioengineering, synthetic biology, and drug delivery. In this paper, we report how the reversible photoswitching of synthetic lipid bilayer membranes made from azobenzene-containing phosphatidylcholine (azo-PC) molecules (photolipids) leads to increased membrane permeability. We show that cell-sized, giant unilamellar vesicles (GUVs) prepared from photolipids display leakage of fluorescent dyes after irradiation with UV-A and visible light. Langmuir-Blodgett and patch-clamp measurements show that the permeability is the result of transient pore formation. By comparing the trans-to-cis and cis-to-trans isomerization process, we find that this pore formation is the result of area fluctuations and a change of the area cross-section between both photolipid isomers.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Chemie und Pharmazie > Department Chemie
• Themengebiete: 500 Naturwissenschaften und Mathematik > 540 Chemie
• ISSN: 0743-7463
• Sprache: Englisch