Impact of Agrobacterium-infiltration and transient overexpression of BroMYB28 on glucoraphanin biosynthesis in broccoli leaves

Kim, Young-Cheon; Cha, Ahra; Hussain, Muhammad; Lee, Kwanuk und Lee, Sanghyeob (2020): Impact of Agrobacterium-infiltration and transient overexpression of BroMYB28 on glucoraphanin biosynthesis in broccoli leaves. In: Plant Biotechnology Reports, Bd. 14, Nr. 3: S. 373-380

Abstract

To date studies revealed that MYB28 intensively involved biosynthesis of GSLs, there is no direct evidence of MYB28 in glucoraphanin (GR) biosynthesis of broccoli. Furthermore, Agrobacterium (Agro)-infiltration also induced GSLs in Arabidopsis. However, there is no information regarding Agro-dependent GR accumulation in broccoli. We revealed that over-expression of BroMYB28 could accumulate GR, and Agro-infiltration is also enough to induce the accumulation of GR in broccoli. From our results, it is suggested that they used different regulatory mechanisms;BroMYB28 over-expression induced both BroMYB28 and BroMYB29 (Bol008849-like) transcripts;Agro-infiltration induced only BroMYB29 (Bol008849-like) transcripts.

• Dokumententyp: Zeitschriftenartikel
• Fakultät: Biologie > Department Biologie I
• Themengebiete: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
• ISSN: 1863-5466
• Sprache: Englisch