Impact of Agrobacterium-infiltration and transient overexpression of BroMYB28 on glucoraphanin biosynthesis in broccoli leaves

Kim, Young-Cheon; Cha, Ahra; Hussain, Muhammad; Lee, Kwanuk; Lee, Sanghyeob (2020): Impact of Agrobacterium-infiltration and transient overexpression of BroMYB28 on glucoraphanin biosynthesis in broccoli leaves. In: Plant Biotechnology Reports, Vol. 14, No. 3: pp. 373-380

Abstract

To date studies revealed that MYB28 intensively involved biosynthesis of GSLs, there is no direct evidence of MYB28 in glucoraphanin (GR) biosynthesis of broccoli. Furthermore, Agrobacterium (Agro)-infiltration also induced GSLs in Arabidopsis. However, there is no information regarding Agro-dependent GR accumulation in broccoli. We revealed that over-expression of BroMYB28 could accumulate GR, and Agro-infiltration is also enough to induce the accumulation of GR in broccoli. From our results, it is suggested that they used different regulatory mechanisms;BroMYB28 over-expression induced both BroMYB28 and BroMYB29 (Bol008849-like) transcripts;Agro-infiltration induced only BroMYB29 (Bol008849-like) transcripts.

• Item Type: Journal article
• Faculties: Biology > Department Biology I
• Subjects: 500 Science > 570 Life sciences; biology
• ISSN: 1863-5466
• Language: English