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Kim, Young-Cheon; Cha, Ahra; Hussain, Muhammad; Lee, Kwanuk; Lee, Sanghyeob (2020): Impact of Agrobacterium-infiltration and transient overexpression of BroMYB28 on glucoraphanin biosynthesis in broccoli leaves. In: Plant Biotechnology Reports, Vol. 14, No. 3: pp. 373-380
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Abstract

To date studies revealed that MYB28 intensively involved biosynthesis of GSLs, there is no direct evidence of MYB28 in glucoraphanin (GR) biosynthesis of broccoli. Furthermore, Agrobacterium (Agro)-infiltration also induced GSLs in Arabidopsis. However, there is no information regarding Agro-dependent GR accumulation in broccoli. We revealed that over-expression of BroMYB28 could accumulate GR, and Agro-infiltration is also enough to induce the accumulation of GR in broccoli. From our results, it is suggested that they used different regulatory mechanisms;BroMYB28 over-expression induced both BroMYB28 and BroMYB29 (Bol008849-like) transcripts;Agro-infiltration induced only BroMYB29 (Bol008849-like) transcripts.