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Wierbowski, Shayne D.; Vo, Tommy V.; Falter-Braun, Pascal; Jobe, Timothy O.; Kruse, Lars H.; Wei, Xiaomu; Liang, Jin; Meyer, Michael J.; Akturk, Nurten; Rivera-Erick, Christen A.; Cordero, Nicolas A.; Paramo, Mauricio I.; Shayhidin, Elnur E.; Bertolotti, Marta; Tippens, Nathaniel D.; Akther, Kazi; Sharma, Rita; Katayose, Yuichi; Salehi-Ashtiani, Kourosh; Hao, Tong; Ronald, Pamela C.; Ecker, Joseph R.; Schweitzer, Peter A.; Kikuchi, Shoshi; Mizuno, Hiroshi; Hill, David E.; Vidal, Marc; Moghe, Gaurav D.; McCouch, Susan R. and Yu, Haiyuan (2020): A massively parallel barcoded sequencing pipeline enables generation of the first ORFeome and interactome map for rice. In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 117, No. 21: pp. 11836-11842

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Systematic mappings of protein interactome networks have provided invaluable functional information for numerous model organisms. Here we develop PCR-mediated Linkage of barcoded Adapters To nucleic acid Elements for sequencing (PLATE-seq) that serves as a general tool to rapidly sequence thousands of DNA elements. We validate its utility by generating the ORFeome for Oryza sativa covering 2,300 genes and constructing a high-quality protein-protein interactome map consisting of 322 interactions between 289 proteins, expanding the known interactions in rice by roughly 50%. Our work paves the way for high-throughput profiling of protein-protein interactions in a wide range of organisms.

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