Logo Logo
Switch Language to German

Gasperotti, Ana F.; Herrera Seitz, M. Karina; Balmaceda, Rocio S.; Prosa, Luciano M.; Jung, Kirsten and Studdert, Claudia A. (2020): Direct binding of benzoate derivatives to two chemoreceptors with Cache sensor domains in Halomonas titanicae KHS3. In: Molecular Microbiology, Vol. 115, No. 4: pp. 672-683

Full text not available from 'Open Access LMU'.


Halomonas titanicae KHS3, isolated from a hydrocarbon-contaminated sea harbor in Argentina, is able to grow on aromatic hydrocarbons and displays chemotaxis toward those compounds. This behavior might contribute to the efficiency of its degradation capacity. Using high throughput screening, we identified two chemoreceptors (Htc1 and Htc2) that bind benzoate derivatives and other organic acids. Whereas Htc1 has a high affinity for benzoate (K-d 112 mu M) and 2-hydroxybenzoate (K-d 83 mu M), Htc2 binds 2-hydroxybenzoate with low affinity (K-d 3.25 mM), and also C3/C4 dicarboxylates. Both chemoreceptors are able to trigger a chemotactic response of E. coli cells to the specific ligands. A H. titanicae htc1 mutant has reduced chemotaxis toward benzoate, and is complemented upon expression of the corresponding receptor. Both chemoreceptors have a Cache-type sensor domain, double (Htc1) or single (Htc2), and their ability to bind aromatic compounds is reported here for the first time.

Actions (login required)

View Item View Item