Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida)

Steinert, Georg; Busch, Kathrin; Bayer, Kristina; Kodami, Sahar; Arbizu, Pedro Martinez; Kelly, Michelle; Mills, Sadie; Erpenbeck, Dirk ; Dohrmann, Martin; Wörheide, Gert ; Hentschel, Ute und Schupp, Peter J. (24. April 2020): Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida). In: Frontiers in Microbiology, Bd. 11, 716: S. 1-16

Abstract

In the present study, we profiled bacterial and archaeal communities from 13 phylogenetically diverse deep-sea sponge species (Demospongiae and Hexactinellida) from the South Pacific by 16S rRNA-gene amplicon sequencing. Additionally, the associated bacteria and archaea were quantified by real-time qPCR. Our results show that bacterial communities from the deep-sea sponges are mostly host-species specific similar to what has been observed for shallow-water demosponges. The archaeal deep-sea sponge community structures are different from the bacterial community structures in that they are almost completely dominated by a single family, which are the ammonia-oxidizing genera within the Nitrosopumilaceae. Remarkably, the archaeal communities are mostly specific to individual sponges (rather than sponge-species), and this observation applies to both hexactinellids and demosponges. Finally, archaeal 16s gene numbers, as detected by quantitative real-time PCR, were up to three orders of magnitude higher than in shallow-water sponges, highlighting the importance of the archaea for deep-sea sponges in general.

• Dokumententyp: Zeitschriftenartikel
• Publikationsform: Publisher's Version
• Keywords: 16S rRNA amplicons; archaea; bacteria; Demospongiae; Hexactinellida; Porifera; quantitative real-time PCR (qPCR); South Pacific Ocean
• Fakultät: Geowissenschaften > Department für Geo- und Umweltwissenschaften
• Themengebiete: 500 Naturwissenschaften und Mathematik > 550 Geowissenschaften, Geologie
• ISSN: 1664-302X
• Sprache: Englisch