Abstract
Conjugation of proteins to AMP (AMPylation) is a prevalent post-translational modification (PTM) in human cells, involved in the regulation of unfolded protein response and neural development. Here we present a tailored pronucleotide probe suitable for in situ imaging and chemical proteomics profiling of AMPylated proteins. Using straightforward strain-promoted azide-alkyne click chemistry, the probe provides stable fluorescence labelling in living cells.
Item Type: | Journal article |
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Research Centers: | Graduate School of Systemic Neurosciences (GSN) |
Subjects: | 500 Science > 500 Science |
ISSN: | 1439-4227 |
Language: | English |
Item ID: | 90648 |
Date Deposited: | 25. Jan 2022, 09:36 |
Last Modified: | 25. Jan 2022, 09:36 |