ECE2 regulates neurogenesis and neuronal migration during human cortical development

Buchsbaum, Isabel Y.; Kielkowski, Pavel; Giorgio, Grazia; O'Neill, Adam C.; Di Giaimo, Rossella; Kyrousi, Christina; Khattak, Shahryar; Sieber, Stephan A.; Robertson, Stephen P. und Cappello, Silvia (2020): ECE2 regulates neurogenesis and neuronal migration during human cortical development. In: EMBO Reports, Bd. 21, Nr. 5, e48204

Abstract

During embryonic development, excitatory projection neurons migrate in the cerebral cortex giving rise to organised layers. Periventricular heterotopia (PH) is a group of aetiologically heterogeneous disorders in which a subpopulation of newborn projection neurons fails to initiate their radial migration to the cortex, ultimately resulting in bands or nodules of grey matter lining the lateral ventricles. Although a number of genes have been implicated in its cause, currently they only satisfactorily explain the pathogenesis of the condition for 50% of patients. Novel gene discovery is complicated by the extreme genetic heterogeneity recently described to underlie its cause. Here, we study the neurodevelopmental role of endothelin-converting enzyme-2 (ECE2) for which two biallelic variants have been identified in two separate patients with PH. Our results show that manipulation of ECE2 levels in human cerebral organoids and in the developing mouse cortex leads to ectopic localisation of neural progenitors and neurons. We uncover the role of ECE2 in neurogenesis, and mechanistically, we identify its involvement in the generation and secretion of extracellular matrix proteins in addition to cytoskeleton and adhesion.

• Dokumententyp: Zeitschriftenartikel
• Fakultätsübergreifende Einrichtungen: Graduate School of Systemic Neurosciences (GSN)
• Themengebiete: 500 Naturwissenschaften und Mathematik > 500 Naturwissenschaften
• ISSN: 1469-221X
• Sprache: Englisch